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Measuring sub-nanometre thickness changes during phase transitions of supported lipid bilayers with quantitative differential interference contrast microscopy

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posted on 2024-09-19, 08:33 authored by David ReganDavid Regan, Joseph WilliamsJoseph Williams, Francesco MasiaFrancesco Masia, Paola BorriPaola Borri, Wolfgang LangbeinWolfgang Langbein

The data contains both fluorescence and DIC images in .tiff format. Minimised qDIC phase images are also included as .tiff files, along with the raw qDIC phase output as .dat files. Measurements of supported lipid bilayer steps are included as ASCII files, in which the first column is the bilayer thickness (nm) and the second column (if present) is the corresponding fluorescence step (arbitrary units). A HTML file is included with full descriptions of all data.

Research results based upon these data are published at https://doi.org/10.1117/12.2510461

Funding

Multiphoton microscopy of lipid-protein dynamics in living cells using correlative Coherent Antistokes Raman Scattering and Two-Photon Fluorescence

Biotechnology and Biological Sciences Research Council

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Quantitative spatio-temporal study of single membrane proteins and lipid domains with label-free super-resolution optical microscopy (2015-10-01 - 2019-03-31); Regan, David. Funder: Engineering and Physical Sciences Research Council

Quantitative label-free optical microscopy of nanostructures (2015-10-01 - 2019-03-31); Williams, Joseph. Funder: Engineering and Physical Sciences Research Council

History

Language(s) in dataset

English-Great Britain (EN-GB)

Data-collection start date

2016-10-27

Data-collection end date

2018-09-28

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Keywords

Lipid raft Optics - Interferometry Fluorophores Bilayers Membrane biophysics

Licence

CC BY 4.0

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