Data for the paper in Applied Surface Science "Antimicrobial release from a lipid bilayer titanium implant coating is triggered by Staphylococcus aureus alpha-haemolysin"

Source data for the paper in the journal " Applied Surface Science": 
Figure 1A-1C.xls - quartz crystal microbalance (QCM-I) data for deposition of novobiocin without PC lipid/cholesterol (NVB/Ti), PC lipid/cholesterol without novobiocin (Lipid/Ti) and novobiocin encapsulated within a PC lipid /cholesterol  (NVB/SLB/Ti) on the surface of octadecylphosphonic acid (ODPA)-modified Ti-QCM sensor

Figure 1C statistics.xls - Water contact angle values and statistical analysis for Ti, ODPA-modified Ti, PC lipid/cholesterol -Ti and novobiocin encapsulated within a PC lipid /cholesterol.

Figure 2B.xls - Histogram of height distribution on the ODPA-coated Ti surface (ODPA/Ti)

Figure 2D.xls - Height profiles of the horizontal white lines in Figure 2C (source image) for ODPA deposited on the Ti-QCM sensor (ODPA/Ti).

Figure 3.xls - CCK-8 assay to determine the cytotoxicity of hBMSCs cultured for 24, 48, 72, 96 hours on culture plastic, Ti, ODPA-modified Ti (ODPA/Ti), lipid coated Ti (Lipid/Ti), Ti coated with supported lipid bilayer encapsulating novobiocin (NVB/SLB/Ti). 

Figure 3 statistics.xls - statistical analysis for Figure 3 (CCK-8 assay to determine the cytotoxicity of hBMSCs)

Figure 4A-4B.xls - (A) Horse blood haemolysis test using different concentrations of α-haemolysin (0.1-10 μg/mL) demonstrates 10 μg/mL of purified α-haemolysin is required to lyse horse erythrocytes. The negative control was horse blood treated with PBS whilst the positive control was horse blood treated with triton x-100. Data representing three experiments (B) Release of 6-FAM from supported lipid bilayers (SLBs) on the surface of Ti discs triggered by different concentrations of α-haemolysin (0.1-10 μg/mL) at 24, 48, 72, 96 hours.

Figure 4A statistics.xls - statistical analysis for Figure 4A (Horse blood haemolysis test)

Figure 5B-5C.xls - B. Semi-quantification of percentage area covered by S. aureus attached to sample surfaces: Ti samples (Ti), S. aureus on the surface of SLBs without novobiocin on the surface of ODPA-modified Ti discs (Lipid/Ti), SLB with encapsulated novobiocin on the surface of ODPA-modified Ti discs (NVB/SLB/Ti) and Ti samples treated with 70% propanol at a magnification x10, x20 and x40. C. Ratio of live to dead bacteria on the surface of samples for fluorescent live/dead images of S. aureus attached to sample surfaces: Ti samples (Ti), S. aureus on the surface of SLBs without novobiocin on the surface of ODPA-modified Ti discs (Lipid/Ti), SLB with encapsulated novobiocin on the surface of ODPA-modified Ti discs (NVB/SLB/Ti) and Ti samples treated with 70% propanol at a magnification x10, x20 and x40. 

Figure 5B statistics.xls - statistical analysis for Figure 5B (Semi-quantification of percentage area covered by S. aureus)

Figure 5C statistics.xls - statistical analysis for Figure 5C (Ratio of live to dead bacteria on the surface of samples)

Source images for the same paper:

Figure 1B jpg - images water contact angle test of the modified Ti disc surfaces immediately after UV/ozone cleaning (Ti); after modification with ODPA (ODPA/Ti); after a PC/cholesterol SLB (Lipid/Ti) was deposited on the ODPA-coated Ti surface; and the Ti disc modified with a SLB with encapsulated novobiocin (NVB/SLB/Ti).

Figure 1D jpg - Fluorescence microscopy images of SLB developed on the surface of ODPA-modified Ti-QCM sensors before and after 10 minutes of incubation in SDS solution at magnifications of x10, x20 and x40. Data representative of three experiments

Figure 2A jpg - AFM images of ODPA deposited on the Ti-QCM sensor (ODPA/Ti).

Figure 2B tif - image of histogram of height distribution on the ODPA-coated Ti surface (ODPA/Ti).

Figure 2C tif - AFM height images of ODPA-modified Ti.

Figure 2D tif - image of height profiles of the horizontal white lines in 2C for ODPA deposited on the Ti-QCM sensor (ODPA/Ti).

Figure 3 tif - image of CCK-8 assay to determine the cytotoxicity of hBMSCs cultured for 24, 48, 72, 96 hours on culture plastic, Ti, ODPA-modified Ti (ODPA/Ti), lipid coated Ti (Lipid/Ti), Ti coated with supported lipid bilayer encapsulating novobiocin (NVB/SLB/Ti).

Figure 4A jpg - image for horse blood haemolysis test using different concentrations of α-haemolysin (0.1-10 μg/mL) demonstrates 10 μg/mL of purified α-haemolysin is required to lyse horse erythrocytes. The negative control was horse blood treated with PBS whilst the positive control was horse blood treated with triton x-100.

Figure 4B jpg - image for release of 6-FAM from SLBs on the surface of Ti discs triggered by different concentrations of α-haemolysin (0.1-10 μg/mL) at 24, 48, 72, 96 hours.

Figures 5A jpg - Fluorescent live/dead images of S. aureus attached to sample surfaces. The green signal indicates viable bacteria whilst the red signal indicates non-viable bacteria. S. aureus on the surface of Ti samples (Ti), S. aureus on the surface of SLBs without novobiocin on the surface of ODPA-modified Ti discs (Lipid/Ti), SLB with encapsulated novobiocin on the surface of ODPA-modified Ti discs (NVB/SLB/Ti) and Ti samples treated with 70% propanol at a magnification x10, x20 and x40.