Data for publication: Modification of the antibiotic, colistin, with dextrin causes enhanced cytotoxicity and triggers apoptosis in myeloid leukemia

The dataset relates to figures presented in a research article published in bioRxiv and International Journal of Nanomedicine with the same title and authors. The data in each tab shows:

"Fig. 1": Absorbance values of 96-well plate wells containing red blood cells incubated with colistin sulfate, dextrin-colistin conjugates, PBS or Triton X-100 for 24 h at 37^oC. Table shows data from repeat experiments for each treatment. Units are arbitrary. Columns represent different drug concentrations/treatments and rows represent technical replicates.

"Fig. 2": Fluorescence and luminescence values of 96-well plate wells containing cells incubated with controls, colistin sulfate or dextrin-colistin conjugates for 24 h at 37^oC that have undergone CellTiter blue, Cytotox-One and Caspase-Glo 3/7 assays. Table shows data from repeat experiments for each treatment. Units are arbitrary. Columns represent different drug concentrations/treatments and rows represent technical replicates.

"Fig. 3": Fluorescence and luminescence values of 96-well plate wells containing cells incubated with controls, colistin sulfate or dextrin-colistin conjugates for 24 h at 37^oC that have undergone CellTiter blue, Caspase-Glo 3/7 and Realtime-Glo Annexin V apoptosis and necrosis assays. Table shows data from repeat experiments for each treatment. Units are arbitrary. Columns represent different drug concentrations/treatments and rows represent technical replicates.

"Fig. 4": Panel a) Percentage of live cells at each stage of the cell cycle, detected by flow cytometry with fluorescence detection. Units are %. Columns represent the different cell cycle phases and rows represent different drug concentrations/treatments. Data from repeat experiments are shown in seperate tables. Panels b,c) Number of colonies counted in a 35 mm culture dish after 10-day incubation of cells with vehicle, colistin sulfate or dextrin-colistin conjugate. Units are number of colonies. Columns represent technical replicates and rows represent different drug concentrations/treatments.

"Fig. 5": Percentage of live cells cells incubated with controls, colistin sulfate or dextrin-colistin conjugates for 2 h at 4 and 37^oC within the gated area corresponding to parent (untreated) cells, with corresponding mean fluorescence. Rows within each treatment group shows biological replicates and columns show data from technical replicates.

"Fig. S1": Absorbance values of chromatography analysis during dextrin-colistin conjugate purification by FPLC. Rows show absorbance at different elution volumes and columns show absorbance at 210 and 280 nm, as well as identification of fractions collected and pooled. Each table represents data for the different conjugates used in the study. Units are arbitrary.

"Fig. S2": Absorbance values (at 210 and 280 nm) of chromatography analysis of colistin sulfate and dextrin-colistin conjugate by FPLC. Column A shows elution/fraction volume, column B shows absorbance of fractions following analysis by BCA assay and columns C-F show absorbance of dextrin-colistin conjugate and colistin sulfate. Units are arbitrary.

"Fig. S3": Fluorescence and luminescence values of 96-well plate wells containing cells incubated with controls, colistin sulfate or dextrin-colistin conjugates for 72 h at 37^oC that have undergone CellTiter blue, Cytotox-One and Caspase-Glo 3/7 assays. Table shows data from repeat experiments for each treatment. Units are arbitrary. Columns represent different drug concentrations/treatments and rows represent technical replicates.

"Fig. S4": Fluorescence and luminescence values of 96-well plate wells containing cells incubated with controls, colistin sulfate or dextrin-colistin conjugates for 24 h at 37^oC that have undergone CellTiter blue and Caspase-Glo 3/7 assays. Table shows data from repeat experiments for each treatment. Units are arbitrary. Columns represent different drug concentrations/treatments and rows represent technical replicates.

"Fig. S5": Fluorescence values of collected fractions following PD-10 seperation of fluorescent probes. Column A shows elution/fraction volume, columns B-D show fluorescence of fractions (measured by platereader) and column E shows absorbance following analysis by BCA assay. Units are arbitrary.

"Fig. S6": Percentage of live cells cells incubated with controls, colistin sulfate or dextrin-colistin conjugates for 2 h at 4 and 37^oC within the gated area corresponding to parent (untreated) cells, with corresponding mean fluorescence. Rows within each treatment group shows biological replicates and columns show data from technical replicates.