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Correlative light electron microscopy using small gold nanoparticles as single probes - data

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posted on 2024-09-18, 11:35 authored by Iestyn PopeIestyn Pope, Hugh Tanner, Francesco MasiaFrancesco Masia, Lukas PayneLukas Payne, Kenton P Arkill, Judith Mantell, Wolfgang LangbeinWolfgang Langbein, Paul Verkade, Paola BorriPaola Borri

The data consists of images acquired with optical microscopy and electron microscopy, and associated quantitative analysis. Images show the spatial distribution of gold nanoparticles inside biological cells. They are provided with scale bars and intensity scales, in a power point file.

Associated analysis plots indicate the measured amplitude and phase of the detected nonlinear light field (four-wave mixing) versus the the gold particle aspect ratio and orientation. They are provided as Origin graphs, indicating x,y scales and units. 

A video is also provided as .mov file, showing a reconstructed electron tomography from a tilt series of the section from -55 to +55 degrees and 1 degree increments.

Research results based upon these data are published at http://doi.org/10.1038/s41377-023-01115-4


Funding

Cardiff University Experimental Equipment

Engineering and Physical Sciences Research Council

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Shedding new light on cells with coherent multiphoton nanoscopy

Engineering and Physical Sciences Research Council

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Nonlinear plasmonic biosensing and functional imaging

Engineering and Physical Sciences Research Council

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History

Specialist software required to view data files

Power Point, Origin, Apple QuickTime Player (Mac), Microsoft Windows Media Player (Windows)

Language(s) in dataset

  • English-Great Britain (EN-GB)

Data-collection start date

2016-01-01

Data-collection end date

2022-09-01

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    School of Biosciences

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