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Confocal 3D scleral fibroblast cytoskeleton - data

dataset

posted on 2024-09-18, 10:54 authored by Petar MarkovPetar Markov, Anthony HayesAnthony Hayes, Hanxing ZhuHanxing Zhu, Craig BooteCraig Boote, Emma BlainEmma Blain

Confocal 3D cytoskeleton datasets (primary bovine scleral fibroblasts either left unloaded or exposed to physiological tensile strain and imaged 1, 6 or 24hrs post-cessation of strain) and labelled for F-actin, tubulin microtubules, vimentin intermediate filaments and cell nuclei.

Research results based upon these data are published at https://doi.org/10.1002/jbio.202000202

Funding

Cellular and extracellular aspects of posterior eye microstructure and biomechanics (2016-10-01 - 2020-04-30); Markov, Petar. Funder: Engineering and Physical Sciences Research Council

DTP 2016-17 Cardiff University (2016-10-01 - 2021-09-30); Phillips, Rhian. Funder: Engineering and Physical Sciences Research Council

History

Specialist software required to view data files

No for TIF, Yes for ROI (ImageJ), No for XLS (but you require MATLAB if you want to run the analysis, along with the script)

Language(s) in dataset

English-Great Britain (EN-GB)

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Keywords

Confocal microscopy Mechanical properties of cells mechanobiology 3D confocal reconstructions cytoskeleton scleral fibroblast

Licence

CC BY 4.0

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